Growth and Production Comparison of Red and Granola Potato (Solanum tuberosum) Microtubers as Influenced by Light Treatment and Different Sucrose Concentrations

Diky Setya Diningrat; Adelia Febriyossa; Selvia Dewi Pohan; Heppy Setya Prima; Larasati Arum Utami; Eka Dodi Suryanto; Bagoes  Maulana; Ayu Nirmala Sari; Asmin

doi:10.55230/mabjournal.v55i2.3555

Authors

Diky Setya Diningrat
dikysd@unimed.ac.id
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, 20221, Indonesia
Adelia Febriyossa Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, 20221, Indonesia https://orcid.org/0009-0003-0117-3384
Selvia Dewi Pohan Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, 20221, Indonesia https://orcid.org/0000-0003-0114-6442
Heppy Setya Prima Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, 20221, Indonesia https://orcid.org/0000-0003-4099-3732
Larasati Arum Utami Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, 20221, Indonesia https://orcid.org/0009-0005-6447-2482
Eka Dodi Suryanto Department of Electrical Engineering, Faculty of Engineering, Universitas Negeri Medan, 20221, Indonesia
Bagoes Maulana Department of Electrical Engineering, Faculty of Engineering, Universitas Negeri Medan, 20221, Indonesia
Ayu Nirmala Sari Department of Biology, Faculty of Sciences and Technology, Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia
Asmin Department of Mathematics, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, 20221, Indonesia

Keywords:

Granola, light treatment, microtubers, red potato, sucrose

Abstract

Microtuber production through tissue culture offers an effective solution for producing high-quality, disease-free potato seed tubers. This study investigates the effects of light treatment and sucrose concentration on microtuber production in red and Granola potato varieties. The research was conducted at the Tissue Culture Laboratory of PT. G10 Agrotechnology in Medan, North Sumatra. The treatments applied were lighting regulation (bright with 1000 lux LED lighting for 24 hr vs. total darkness (0 lux, culture bottles wrapped with aluminum foil) in the incubation room and different sucrose concentrations (80, 100 & 120 g/L) added to the MS (Murashige & Skoog) basic medium. This research used a Completely Randomized Design method with 5 replications. Data were analyzed using ANOVA. Results showed that bright light treatment and 80 g/L sucrose concentration provided the best results for Granola and red potato plantlet growth from 14 to 70 days after planting. For the number, weight, and diameter of microtubers, the combination of dark light treatment and 120 g/L sucrose concentration produced the highest average values for both Granola and red potatoes. Overall, the growth and production of Granola potatoes were higher than those of red potatoes in every parameter. The results of this research can be used as a reference for producing red potato and Granola microtubers through tissue culture.

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